LyoPlas N – w: Preparing for Transfusion
1. Connect transfer set to water bag
Close the clamp on the transfer set. Connect the transfer set to the bag with water for injection (Aqua ad iniectabilia) using a Luer lock connector.
2. Pierce the plasma bottle
Remove the protective cap from the bottle with the freeze-dried plasma. Disinfect the bottle stopper. Push the spike of the transfer set through the centre of the rubber stopper.
The unit consisting of water bag, transfer set and glass bottle is now stable enough to hang freely.
4. Reconstitute the plasma
Gently swirl the bottle to dissolve the freeze-dried plasma. Do not shake. Avoid foaming. The plasma is ready for use when all solid particles are dissolved.
3. Transfer the water
Open the clamp and allow the water to completely flow from the bag into the glass bottle. (IMPORTANT: Do not press the bag as this will block the air filter!) Close the clamp.
Remove the transfer set with the bag. Suspend the bottle by its hanger label. An administration set with air vent and with a 170-230 µm standard filter is required for transfusion.
5.b Mark the bag with the service label
Keep the bag set and glass bottle together to begin with. Peel the service label off the plasma bottle and use it to mark the bag.
6. Transfer the plasma from the bottle to the bag
Suspend the bag by its hanger label, open the clamp. Allow the reconstituted plasma to flow into the bag.
7. Evacuate the air
Evacuate the air from the bag; this is particularly important before pressure transfusion takes place. Gently press the plasma bag so that the air above the plasma can escape via the transfer set. Then close the clamp.
Check the bar code numbers to ensure correct identification. Only then should the transfer set be disconnected from the bag. An administration set with a 170-230 µm standard filter should be used for transfusion. (An air vent is not required if transfusion is made from the bag.)